Oil Spill Cleanup using Bioremediation

Proven Oil Spill Cleanup Technology

Ship Oil Spill
Oil Spill Cleanup

BioWorld has achieved a high degree of success with our bioremediation technology. Since 1972, bioremediation has now matured to an important economical method of remediating soils and groundwater. Much of the early work on bioremediation involved adding fertilizers in an attempt to improve natural processes. BioWorld's technology provides consistent results that were lacking in the past.

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Bioremediation Contact Button

What To Expect When You Contact Us:

  1. A response from one of our knowledgeable technicians within one business day of your enquiry.
  2. Customized solutions to your problems created by our technicians designed specifically for your needs.
  3. Technical support to ensure that our products provide you with the best solution possible.

Effective Integrated Approach Using
Biotechnology, Chemistry, and Engineering

BioWorld bioremediation products and services provide an efficient, environmentally safe, on-site treatment solution to many hazardous waste cleanups. We restore most contaminated sites in less time and at a far lower cost than other remediation techniques.

BioWorld Emergency Response

BioWorld Hydrocarbon Treatment products are listed on the EPA National Contingency Plan Product Schedule and the California Office of Spill Prevention and Response for oil spill cleanups.

What is BioWorld Hydrocarbon Treatment?

BioWorld Oil Spill Cleanup Bioremediation Product


BioWorld Bioremediation is a natural and completely safe 2-part product consisting of the following:

  1. Bioenhancement - Bioremediation Enhancer liquid formulation which maximizes the ability of microbes to reproduce and thrive in a hazardous waste situation (in soil or water applications)
  2. Bioaugmentation - Hydrocarbon Digesting Microbes in a dry form for remediating petroleum hydrocarbon waste.

When applied to oil spills, fuel spills, oily water and other petroleum hydrocarbon contamination, BioWorld Hydrocarbon Treatment effectively digests hydrocarbon waste while producing non-hazardous by-products. To date, our products and technology have been used to clean up crude oil, gasoline, diesel, MTBE and other petrochemicals.

Depending on the project type, conditions, initial contaminant concentration and clean-up target, BioWorld Bioremediation is added to the soil or water using in situ or ex situ techniques. BioWorld provides the framework for the microbes to directly metabolize contaminants and create enzymes for efficient conversion of contaminants to water, carbon dioxide and other non-hazardous substances such as fatty acids.

Native soil have many types of tiny creatures called microbes.

When hazardous wastes contaminate the soil, the existing microbes struggle to survive. Strains known to be efficient at degrading the waste may or may not be present. In addition, the microbes generally lack the proper nutrients to reproduce and thrive.

Using BioWorld Bioremediation Technology, the proper strains of selected microbes and soluble forms of the necessary nutrients are easily applied to the waste situation.

Billions of healthy microbes constantly work to break down hazardous waste into harmless by-products.

A - BioWorld provides an efficient, safe for the environment, on-site treatment solution to many hazardous waste cleanups.

B - Long-term liability from off-site transport and disposal is completely eliminated.

The result is a successful, cost-effective cleanup in compliance with environmental regulations.


Contact BioWorld for more information

EPA National Contingency Plan Product Schedule Listing

BioWorld Hydrocarbon Treatment is listed on the EPA NCP Product Schedule under Bioremediation Agents. This enables federal on-scene coordinators to authorize the use of BioWorld for oil spill cleanups.

State of California OSPR Licensed

BioWorld Hydrocarbon Treatment is licensed by the State of California, Department of Fish and Game, Office of Spill Prevention and Response as an Oil Spill Cleanup Agent. This enables the State Regional Response Team to approve BioWorld to be used for oil spill cleanups in California.

Certified US Government Supplier

BioWorld has earned two GSA Contracts - one for Environmental Services and one for products. GSA has scrutinized BioWorld's prices, references, company history and ability to provide remediation services and products. By using BioWorld's GSA contract privilege, formal bid proposals can be avoided. BioWorld simplifies the process of procuring remediation services and products.

The On-Site Solution

A significant advantage of using BioWorld Bioremediation is conducting the remediation on-site. The need to transport hydrocarbon contaminated soil or water off-site as hazardous waste is nonexistent. Permits and/or licenses necessary to transport contaminants is eliminated saving you time and money.

Eliminate Liability

As long as contamination exists, the property owner is legally and financially responsible from cradle to grave - whether or not the contamination has been moved off-site. BioWorld Bioremediation digests the hydrocarbon contamination in soil or water and produces harmless by-products such as carbon dioxide, water, fatty acids and biomass. Once the contamination problem is resolved, liability is removed.

More Than a Decade of Expertise

For more than 15 years, BioWorld Bioremediation technology has proven to be a non-disruptive, cost-effective and highly efficient method of safely breaking down many chemicals. Our 2-part enhancement and augmentation approach results in a faster and more complete degradation of petroleum hydrocarbon contamination in surface soils, subsurface soils, groundwater, surface waters or virtually any other area.

Other Advantages

  • Faster and more effective cleanup over natural attenuation processes
  • Environmentally safe and natural approach
  • Easy to apply - no protective clothing required
  • Cost effective and efficient methods for saltwater, freshwater, soil, sand or other surfaces.

For additional information, Contact Us
Phone: (559) 651-2042

MSDS Information

Printable PDF


Application Instructions Small Scale Projects

General application is 1 gallon Bioremediation Enhancer and 1/2 lb Hydrocarbon Degrader Microbes per 2,000 square feet of contaminated surface soil with an average Total Petroleum Hydrocarbon concentration of 2,000 ppm. Adjust product amounts based on project type (soil or water), initial hydrocarbon concentration and target level for cleanup. Contact BioWorld for large scale projects or your project specific requirements.

Preparing Microbe Solution

  1. In a 5 to 6 gallon bucket, add 1/2 to 1 unit BioWorld Hydrocarbon Degrader Microbes to several gallons of 100° - 105° F warm water. The result is a brownish solution of microorganisms which are transitioning from their dormant state.
  2. Mix vigorously and let stand uncovered for 5-10 minutes to reactivate the microbes while stirring periodically. Use the microbe solution within 4 hours and keep unsealed.

Preparing Optimizer Solution (Blue Liquid)

  1. Pour 1/2 gallon of BioWorld Bioremediation Enhancer into the sprayer. (Keep separate from the microbe bucket).

Application Procedure

  1. Evenly spray the Bioremediation Enhancer (blue liquid) directly onto the oil contaminated area until 1/2 gallon of product is used up.
  2. Add the Microbe Solution (brownish liquid) from the pail directly onto the oil contaminated area until used up.
  3. Work the products into the soil with a shovel or rake. Break up and turn over the surface soil and apply water to moisten the area (target of 20% moisture at a minimum).
  4. Apply water to the soil every week or more often as needed.
  5. Turn over the treated soil every week or more often as needed. Keep the treated soil moist and loose.
  6. Re-apply the BioWorld products as needed.

Product Specifications

Print Version

BioWorld Bioremediation Treatment uses a unique combination of bioenhancement compounds and mature strains of selected, naturally occurring microorganisms for the bioremediation of petrochemical wastes.

BioWorld utilizes the following two technologies:

  1. Bioenhancement - The special Bioremediation Enhancer formulation maximizes the ability of the microbes to reproduce and thrive in the waste system.
  2. Bioaugmentation - Hydrocarbon Digesting Microbes consist of various microbial strains and biochemical nutrients for refinery and petrochemical wastes. BioWorld Bioremediation Treatment is environmentally safe, non-hazardous, non-toxic, non-corrosive, non-flammable, and non-pathogenic.

Bioremediation Enhancer
#75014 - 1 gallon Bioremediation Enhancer
#75064 - 6-gallons Bioremediation Enhancer
#75250 - 250-gallons Bioremediation Enhancer

Hydrocarbon Digesting Microbes
#32086 - 0.5 pound Hydrocarbon Digesting Microbes
#32055 - 2.5 pounds Hydrocarbon Digesting Microbes

Small Project Combinations
#82186 - Hydrocarbon Treatment Unit
(Both consists of 1-gallon Bioremediation Enhancer and 0.5-pound Hydrocarbon Digesting Microbes)

 

Composition and Physical Properties

 

Liquid: Bioenhancement Compounds, Vitamins, Minerals, Amino Acids, Polysaccharides, Esters, Alcohols, Oils, Proteins, Organic Acids, Natural Liquid Enzymes, Organic Surfactants
pH: 5.5 - 6.5
Boiling Point: 212°F/100°C
Freezing Point: 32°F / 0°C
Solubility in Water: 100%
Specific Gravity: 1.0028
Appearance & Odor: Blue-Green; Slight Floral Scent

Dry: Selected strains, natural enzymes on a bran carrier.
Concentration: Maximum viable CFU/g - strain and type dependent
Form:: Free Flowing Powder
Color: Light Brown
Odor: Yeast Like
pH: Neutral
Effective pH Range: 5 - 9
Effective Temperature Range: 33° - 110°F


Bunker �C� Fuel Oil Bioremediation Case Study Old wooden fuel storage tanks were excavated and 6,000 cubic yards of contaminated soil was spread over a 4 acre area in 12 parcels � 1 foot deep Water and BioWorld Bioremediation Technology products were applied to the contaminated soil using the water truck. BioWorld Hydrocarbon Degrading Microbes were activated in warm water prior to application � then added to a water truck. Each of the 12 separate parcels was treated on a monthly basis. Technicians added BioWorld Bioremediation Enhancer to a 4,000 gallon water truck. Close-up of the soil shows water and BioWorld Hydrocarbon Treatment product absorption. www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Bunker C Fuel Oil Bioremediation Case Study - Photos The soil was disced for mixing and aeration on a regular basis. Initial Total Petroleum Hydrocarbon Concentrations in the soil averaged 4,000 ppm. The target for cleanup was 100 ppm which was achieved in 11 months. The soil is covered with native plant life and could be safely used for growing edible crops. Some native plant life begins to inhabit the site as the contaminant is degraded. BioWorld Products Used: 75250 - Bioremediation Enhancer 32055 - Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Bunker C Fuel Oil Bioremediation Case Study - Photos FUEL OIL BIOREMEDIATION CASE STUDY A former #6 fuel oil (Bunker C) underground storage tank area contained approximately 6,000 cubic yards of contaminated soil. Twelve (12) treatment cells were constructed containing 500 cubic yards per cell. Initial Total Petroleum Hydrocarbon (TPH) concentrations in the soil averaged 4,000 ppm. The targeted level for closure was 100 ppm. BioWorld Bioremediation Technology was selected over several methods as the most efficient and costeffective solution for cleanup. Specialized compounds, vitamins, and minerals, in conjunction with hydrocarbon degrading microbes, were added to the cells using a water truck on a monthly basis for 3 months. Mechanical discing of the cells was conducted to provide mixing and aeration. The site is located in a desert region with minimal rainfall. Water was added to maintain a limited amount of moisture in the cells. The cleanup time was secondary to budget. After 11 months, the average TPH concentration was reduced to acceptable regulatory standards. The project is deemed complete with no further clean-up action required. AVERAGE TPH CONCENTRATION VS. TIME 4,000 3,500 3,000 2,500 ppm TPH 2,000 1,500 1,000 500 0 8/5/1994 3/3/1995 6/24/1994 7/15/1994 8/26/1994 9/16/1994 10/7/1994 12/9/1994 1/20/1995 2/10/1995 3/24/1995 4/14/1995 5/5/1995 Sample Results 5/26/1995 10/28/1994 11/18/1994 DATE www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Fuel Oil Bioremediation Case Study - Graph 12/30/1994 6/16/1995 10 TPH In-Situ Bioremediation of Gasoline Contaminated Groundwater Case Study Underground storage tanks were removed from an old service station in the mid 1990's. Gasoline had leaked out of the tanks and contaminated the shallow groundwater. An engineering firm was hired to begin the cleanup and 6 monitoring wells were installed. After 7 years of monitoring, there were no changes in the analytical tests and the firm was released. BioWorld teamed up with a progressive and innovative engineering firm determined to clean up the groundwater. BioWorld designed a specialized pump and treat system and procedure using the existing monitoring wells. The site work and cleanup had to be performed with the existing auto sales business operating on the property. BioWorld Bioremediation Enhancer and Hydrocarbon Digesting Microbes were added several times down the monitoring wells. Within 5 days, 8 inches of free floating product had been degraded. Treatment continued for 3 months with a 90% reduction in dissolved phase gasoline constituents verified. BioWorld Products used: 75064 Bioremediation Enhancer 32086 Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved In-Situ Bioremediation of Gasoline Contaminated Groundwater Case Study Mixed Waste Bioremediation Case Study A trucking company regularly washed tanker units to eliminate cross contamination of various liquid products being transported. The mixed waste wash water (vegetable oil and petroleum hydrocarbons) was stored in a metal storage tank prior to discharge into the municipal wastewater system. BioWorld Liquid Optimizer, Hydrocarbon Digesting Microbes and General Blend Microbes were added several times to treat the mixed waste. Note sides of tank � cleaned of sludge after treatment of BioWorld Products. Digestion of the mixed waste was visually apparent and a reduction in TPH and Oil/Grease concentrations was verified. The company saved substantially on wastewater discharge fees. BioWorld Products used: 75014 Bioremediation Enhancer 32086 Hydrocarbon Digester Microbes 36055 General Blend Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Mixed Waste Bioremediation Case Study Nursery Small Scale Bioremediation Case Study A flower production nursery used oil and petroleum hydrocarbon products for their operation. Over time, the soil and concrete in a storage area became contaminated. Since the hydrocarbon contamination area was adjacent to a creek, an on-site inspection by the County Environmental Health Department prompted clean up measures to be implemented. BioWorld provided bioremediation products, application supplies and project management services. BioWorld Bioremediation Enhancer and BioWorld Hydrocarbon Digesting Microbes were added several times to the soil and concrete surfaces. Within 6 weeks, the oily contamination was converted into water, carbon dioxide and non-hazardous substances. The project site was successfully remediated and deemed complete by the regulatory agency for under $1,000. BioWorld Products used: 75014 Bioremediation Enhancer 32086 Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Nursery Small Scale Bioremediation Case Study Oil Sludge Bioremediation Case Study Bench Scale Test Oil Sludge from old well drilling pit. Side by side comparison. (See dried lump in tanks.) Left � Control Right � Treated with BioWorld Hydrocarbon Treatment Products. Several days later. Left (Control) � Water has no signs of biological activity. Right (Treated) � Respiration from microbes degrading the hydrocarbon very apparent. Several days later. Left (Control) � Water has no signs of biological activity. Right (Treated) � Respiration from microbes degrading the hydrocarbon very apparent. Fatty Acids forming on surface as hydrocarbon breaks down. BioWorld Products used: 75014 Bioremediation Enhancer 32086 Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Oil Sludge Bioremediation Case Study - Bench Scale Test Oil/Water Separator Bioremediation Case Study Waste oil was about 3 inches thick in a 600 gallon oil/water separator at a tractor repair facility. 3 days after BioWorld Hydrocarbon Treatment, the oil was degraded for safe disposal as non-hazardous material. 3 days after BioWorld Hydrocarbon Treatment, the thick oil was degraded to a fine layer of fatty acids. (The unit was moved outside.) Oil was about 1 inch thick after treating with BioWorld Hydrocarbon treatment products. The tank was rolled outside. BioWorld saved this client US$400 per month in disposal costs. BioWorld Products Used: 75014 � Bioremediation Enhancer 32086 � Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Oil-Water Separator Bioremediation Case Study diesel r em ediation gasoline rem ediation bun ker o il remed iatio n Woodside Property Bioremediation Case Study Four steel fuel storage tanks (2 diesel, 1 gasoline and 1 bunker oil) were excavated from various locations on a residential property. Soil samples were collected and analyzed for total petroleum hydrocarbons (TPH) which indicated soil contamination was present (the highest TPH concentration was 8,100 mg/kg). The County Health Services Agency requested that the contaminated soil be disposed at an appropriate facility. BioWorld teamed up with an engineering company and proposed bioremediation as a viable option. With the approval of the regulatory agency, a treatment cell was constructed for the 280 cubic yards of stockpiled soils. The contaminated soil was spread out on plastic and contained within a berm to prevent runoff. BioWorld hydrocarbon treatment products and water were added to the soil. The soil was mixed with a rototiller and moisture added as needed. After 2 months, soil samples were collected and analyzed. The results indicated a reduction in TPH concentrations to below state action levels. BioWorld Products used: 75064 Bioremediation Enhancer 32086 Hydrocarbon Digester Microbes www.bioworldusa.com � mail [at] bioworldusa [dot] com � 559.651.2042 International Headquarters � R&D � Manufacturing � Distribution Center 8244 W Hillsdale Ct � PO Box 2920 � Visalia California 93279-2920 � United States of America Copyright � 1991-2006. All rights reserved Woodside Property Case Study

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